Background: Cholelithiasis is a benign disease of the biliary tract is one of the most common indications for major abdominal surgery in India. The most prevalent complication of gallstone is chronic cholecystitis occurs in approximately 4% of cases. Cholelithiasis or gallstones are quite common in adults. Antibiotic prophylaxis in biliary surgery, when correctly used, has led to the reduction in postoperative infections. Cephalosporins are one of the most commonly prescribed agents for surgical prophylaxis because of their favourable pharmacokinetic profiles, low incidence of adverse effects and low costs Methods: Minimum of 50 Patients admitted under General Surgery, who are scheduled to undergo laparoscopic cholecystectomy.Five ml of bile will be aspirated from the gall bladder during laparoscopic cholecystectomy. The samples will be transferred onto a plain sterile container and then transferred at room temperature in an hour to the laboratory for antibiotic sensitivity pattern. Results: Our study revealed that amikacin being the most powerful as its sensitive to e.coli and klebsiella and eneterococcus [100%]. Klebsiella shows complete sensitivity to ampicilin and ciprofloxacin and linezolid are moderately sensitive Ceftriaxone is 100 percent sensitive to klebsiella and 62.5 percent to e.coli Imipenem shows 100 percent sensitivity to enterococci, 33.3% to klebsiella and 50 percent to e. coli. Conclusions: Isolating bacteria and determining their susceptibility to different antibiotics may help physicians to manage cholecystitis and its associated complications. It helps  in reducing the costs associated with health care and help to reserve drugs for future use in case of need of newer microorganisms. Exhausting all available antibiotic options before antibiotic sensitivity will result in paving way for bacterial kingdom to develop a bug which will be resistant to all antibiotics.

Keywords: Gall Stones; Microflora; Culture and Sensitivity; Antibiotics.