Antibacterial resistance is a global public health care concern that is impacted by both human and non human antimicrobial use. The consequences of antimicrobial resistance are particularly important when disease is caused by pathogens that are resistant to antimicrobials considered critically important in the treatment of human disease by the WHO. Fluoroquinolones have been classified as the highest priority drugs by WHO for management of infections due to Enterobacteriaceae. Fluoroquinolones with expanded spectrum of action, excellent tissue penetration and ease of administration (oral) have made them economical and important drugs for OPD practice. Hence study of resistance mechanism is of great importance in the fight against the spread of resistance genes of this class of drugs. The mechanism of Fluoroquinolone resistance is not completely understood and until recently the conventional understanding is that clinically relevant resistance to quinolones in Enterobacteriaceae is always considered to be chromosomal in origin caused by mutation(s) in topoisomerase genes (target enzymes) or affecting drug permeation and transmitted only vertically. Recently the discovery of novel plasmid mediated quinolone resistance (PMQR) mechanism has threatened the diagnostic capabilities of routine diagnostic laboratories and increased the chances of possible spread of resistance by horizontal gene transfer. There is paucity of data regarding PMQR mediated qnr gene in India. Hence the present study was undertaken to study the presence of qnr A and S in Indian isolates and the effect of qnr gene on MIC of various Fluoroquinolones.