Background: Thrombocytopenia is defined as a decrease in peripheral blood platelet count less than 150,000 cells/μL. Immature Platelet Fraction (IPF) is simple non-invasive procedure to measure percentage of reticulated platelets in peripheral blood. Our study aimed at measuring the IPF percentage and evaluating its significance in
hyperdestructive, hypoproductive and megaloblastic thrombocytopenic groups. Methods: In present study a total of 73 cases of thrombocytopenia were studied, which included 43 cases of
hyperdestruction, 16 cases of hypoproduction and 14 cases of megaloblastic group. IPF% along with complete blood counts was estimated for all samples using Sysmex XN350. Cases not meeting the required criteria were excluded from study. Results: Of 73 patients, 56% were females and 44% were males. ITP was the most common clinical diagnosis in the study with 27% of cases followed by dengue and megaloblastic anaemia with 19% each. Out of 43 cases of Hyperdestructive group, 41 (95.3%) cases showed increased IPF in comparison to Hypoproductive group, in which only 01 (0.06%) case out of 16 cases showed increased IPF. Megaloblastic group were in between the both groups showing increased IPF in 06 (42.8%) cases of total 14 cases. Conclusion: Since bone marrow examination is invasive and expensive, platelet indices such as PDW, MPV and P-LCR lack significant sensitivity and specificity, Immature Platelet Fraction can be used as a simple non-invasive procedure for estimating the reticulated platelets percentage in peripheral blood to monitor the megakaryocytic
activity.

Keywords: Immature Platelet Fraction; Megaloblastic Anemia; Reticulated Platelets; Thrombocytopenia.